The new direct service, to launch in May 2026 has been made possible thanks to a collaboration between Denmark's DSB, Germany's Deutsche Bahn (DB) and the Czech Republic's České dráhy (ČD).
Up until now travellers from Copenhagen could reach Prague by changing trains in Berlin - or alternatively a direct flight.
The new Copenhagen-Berlin-Prague route will connect three of Europe's most popular capital cities. The journey time will be around seven hours from Copenhagen to Berlin and around 11 hours from the Danish capital to Prague.
It will include other stops in Germany too with the train passing through Schleswig, Hamburg (Altona, Dammtor and Hauptbahnhof), Berlin (Hauptbahnhof and Südkreuz), Dresden (Neustadt and Hauptbahnhof) and Bad Schandau.
Danish rail operator DSB said that there will be two daily departures, and an extra departure at night time will be available during the summer months.
The route will be operated using modern ComfortJet carriages from ČD, which are designed to offer a first-class travel experience.
"The collaboration with DB and ČD gives us the opportunity to jointly offer a unique and sustainable way to connect three well-visited and attractive European cities - and countries," said Charlotte Kjærulff, Customer Manager at DSB.
"It makes it easier to take the train to experience Europe's rich history, architecture and culture, which we are all quite proud of."
The tickets for the Copenhagen–Berlin–Prague route will be available to purchase six months before the departure date, so in December 2025.
This initiative is part of a broader effort supported by the European Commission to improve cross-border rail connections in Europe. This route is one of ten European pilot projects aimed at promoting international rail transport.
The onboard staff will be local employees from each country through which the train passes, to ensure jobs area created locally.
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